A simple, accurate and rapid reversed phase high performance liquid chromatographic method has been developed and validated for the simultaneous estimation of sildenafil citrate (SIL) and dapoxetine hydrochloride (DPX) in simulated dosage form. The separation was carried out using mobile phase consisting of methanol: 25 mM phosphate buffer (pH, 2.6) (50:50, v/v). The column used was ACE C18, (150 mm x 4.6 mm i.e., 5 µm) with flow rate 1.2 ml/min using PDA detection at 292 nm. The method was linear over a concentration range 30-90 μg/ml for sildenafil citrate (SIL) and 18-54 μg/ml for dapoxetine hydrochloride (DPX) Respectively. The retention time of sildenafil citrateand dapoxetine hydrochloride were found to be 5.2 min and 9.2 min respectively. Results of analysis were validated statistically and by recovery studies. The mean recovery was 99.29±0.54a nd 99.03±0.45for SIL and DPX respectively. The limit of detection (LOD) and the limit of quantification (LOQ) for SIL and DPX were found to be 0.6586 and 1.9956 µg/ml and 0.6169 and 1.8695 µg/ml, respectively. The results of the study showed that the proposed RP-HPLC method was found to be simple, sensitive, precise and accurate and also useful for the routine determination of SIL and DPX in mixture.
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